Modular microfluidics enables kinetic insight from time-resolved cryo-EM
oleh: Märt-Erik Mäeots, Byungjin Lee, Andrea Nans, Seung-Geun Jeong, Mohammad M. N. Esfahani, Shan Ding, Daniel J. Smith, Chang-Soo Lee, Sung Sik Lee, Matthias Peter, Radoslav I. Enchev
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2020-07-01 |
Deskripsi
There is a growing interest in performing time-resolved cryo-EM studies. Here, the authors present a time-resolved sample preparation method for cryo-EM called trEM, which uses a microfluidic device to initiate the biochemical reaction by rapid mixing of the components and then spraying the sample onto a cryo-EM grid to snap-freeze it in a blot-free, automated manner within several milliseconds.