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A floristic investigation was made during 2016 – 2018 to observe the tree diversity and their present status in the Jaldapara National Park (JNP) which is situated in the Duars region of West Bengal, India. A total of 294 species of trees belonging to 189 genera and 63 families, including 4 species of gymnosperms were recorded. Fabaceae (s.l.) with its 36 species has been appeared to be the dominant family. The tree community of JNP was found to be an excellent blend of wild as well as cultivated species of trees. All the four species of gymnosperms recorded are introduced to the park area. The present study highlights on different categories of threatened taxa which contributed about 43.53% of the recorded tree species. Different types of anthropogenic interferences, including NTFP (Non Timber Forest Product) collection and artificial forest-fire causing dangerous threats for the maintenance of its original vegetation and survival of numerous species in all the strata of the vegetation. Strict implementation of forest conservation related rules and the establishment of ex-situ Protected Area near the park may improve the situation.