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Peking Union Medical College sets up a review course of Biochemistry in the general introduction of medicine for the 4+4 clinical medicine pilot class. The mixed classroom design was carried out with Moodle platform to help students with different bachelor education experience to review, consolidate and strengthen their knowledge of Biochemistry as a compulsory course for premedical students. Under the support of Moodle, we created an autonomous learning environment, integrating Macmillan sapling e-learning resources, and establishing a chapter-based pre-class test to improve students' mastery of basic knowledge. Moodle's “Quiz” and “Workshop” were used in case-based learning to help students to link biochemical changes to clinical manifestation of illness. The positive exploration of the teaching mode of Biochemistry by Moodle fits to the training of the students in the pilot class.