FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells
oleh: Srinjan Basu, Lisa-Maria Needham, David Lando, Edward J. R. Taylor, Kai J. Wohlfahrt, Devina Shah, Wayne Boucher, Yi Lei Tan, Lawrence E. Bates, Olga Tkachenko, Julie Cramard, B. Christoffer Lagerholm, Christian Eggeling, Brian Hendrich, Dave Klenerman, Steven F. Lee, Ernest D. Laue
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2018-06-01 |
Deskripsi
Single molecule tracking of fluorescent proteins in live cells is temporally limited by fluorophore photobleaching. Here the authors show using fluorophore pairs that FRET competes with photobleaching to improve photostability and allow longer-term tracking of both single proteins and complexes.