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Proteomic investigation reveals dominant alterations of neutrophil degranulation and mRNA translation pathways in patients with COVID-19
oleh: Renuka Bankar, Kruthi Suvarna, Saicharan Ghantasala, Arghya Banerjee, Deeptarup Biswas, Manisha Choudhury, Viswanthram Palanivel, Akanksha Salkar, Ayushi Verma, Avinash Singh, Amrita Mukherjee, Medha Gayathri J. Pai, Jyotirmoy Roy, Alisha Srivastava, Apoorva Badaya, Sachee Agrawal, Om Shrivastav, Jayanthi Shastri, Sanjeeva Srivastava
Format: | Article |
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Diterbitkan: | Elsevier 2021-03-01 |
Deskripsi
Summary: The altered molecular proteins and pathways in response to COVID-19 infection are still unclear. Here, we performed a comprehensive proteomics-based investigation of nasopharyngeal swab samples from patients with COVID-19 to study the host response by employing simple extraction strategies. Few of the host proteins such as interleukin-6, L-lactate dehydrogenase, C-reactive protein, Ferritin, and aspartate aminotransferase were found to be upregulated only in COVID-19-positive patients using targeted multiple reaction monitoring studies. The most important pathways identified by enrichment analysis were neutrophil degranulation, interleukin-12 signaling pathways, and mRNA translation of proteins thus providing the detailed investigation of host response in COVID-19 infection. Thus, we conclude that mass spectrometry-detected host proteins have a potential for disease severity progression; however, suitable validation strategies should be deployed for the clinical translation. Furthermore, the in silico docking of potential drugs with host proteins involved in the interleukin-12 signaling pathway might aid in COVID-19 therapeutic interventions.