Transcriptional and Post-Transcriptional Polar Effects in Bacterial Gene Deletion Libraries
oleh: André Mateus, Malay Shah, Johannes Hevler, Nils Kurzawa, Jacob Bobonis, Athanasios Typas, Mikhail M. Savitski
| Format: | Article |
|---|---|
| Diterbitkan: | American Society for Microbiology 2021-10-01 |
Deskripsi
Single-gene deletion libraries have allowed genome-wide characterization of gene function and interactions. While each mutant intends to disrupt the function of a single gene, it can unintentionally target other genes, such as those located in the same operon as the deletion.