Conversion of <i>Escherichia coli</i> into Mixotrophic CO<sub>2</sub> Assimilation with Malate and Hydrogen Based on Recombinant Expression of 2-Oxoglutarate:Ferredoxin Oxidoreductase Using Adaptive Laboratory Evolution
oleh: Yu-Chen Cheng, Wei-Han Huang, Shou-Chen Lo, Eugene Huang, En-Pei Isabel Chiang, Chieh-Chen Huang, Ya-Tang Yang
| Format: | Article |
|---|---|
| Diterbitkan: | MDPI AG 2023-01-01 |
Deskripsi
We report the mixotrophic growth of <i>Escherichia coli</i> based on recombinant 2-oxoglutarate:ferredoxin oxidoreductase (OGOR) to assimilate CO<sub>2</sub> using malate as an auxiliary carbon source and hydrogen as an energy source. We employ a long-term (~184 days) two-stage adaptive evolution to convert heterotrophic <i>E. coli</i> into mixotrophic <i>E. coli</i>. In the first stage of evolution with serine, diauxic growth emerges as a prominent feature. At the end of the second stage of evolution with malate, the strain exhibits mixotrophy with CO<sub>2</sub> as an essential substrate for growth. We expect this work will open new possibilities in the utilization of OGOR for microbial CO<sub>2</sub> assimilation and future hydrogen-based electro-microbial conversion.