Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capture
oleh: Philipp Knyphausen, Mariana Rangel Pereira, Paul Brear, Marko Hyvönen, Lutz Jermutus, Florian Hollfelder
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2023-02-01 |
Deskripsi
Custom proteases find applications as therapeutics, in research and in biotechnological applications. Here, the authors establish a protease selection system based on bacterial alpha-2-macroglobulin protease inhibitors and evolve staphylococcal proteases for increased activity and altered specificity.