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In this research, effect of aqueous extract of canola(Brassica napus L. cv Hyola 401) on amount of chl a and b,growth parameters and anatomy of shoot and root of wheat was evaluated. 4-day-old wheat seedlings were treated in Hoagland culture (control) and Hoagland culture with aqueous extract of canola during 8 day.According to our results length of root and stem, dry and fresh weight of shoot and dry weight of root in wheat in presence of aqueous extract  of canola did not decreased significantly.Amount of chl a and b in leaf of wheat in response to aqueous extract increased. Anatomy of stem and root of wheat also was affected in canola aqueous extract and cortex thickness in stem and root  and number of metaxylem decreased.