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Root Cd uptake of durum wheat plants (cv. Beloslava) was characterized in hydroponics conditions. The uptake experiments have been performed in Cd concentration range of 0 – 2 μM adjusted by both stable Cd and radiolabeled (109Cd) tracer. Cd removal from the solution over duration of 1 hour reached 50%. The part of loosely adsorbed Cd ions on root surface accounted for about 20%. Over 30% of absorbed Cd at 0.5 μM Cd treatment was retained in root cell walls. The apparent root Cd accumulation showed concentration-dependant tendency with the highest accumulation value of 7.45 nmol Cd g FW-1.