High-throughput identification of synthetic riboswitches by barcode-free amplicon-sequencing in human cells
oleh: Benjamin Strobel, Maike Spöring, Holger Klein, Dragica Blazevic, Werner Rust, Sergi Sayols, Jörg S. Hartig, Sebastian Kreuz
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2020-02-01 |
Deskripsi
Riboswitches can mediate ligand-dependent RNA cleavage and splicing to control gene expression. Here the authors present a method to functionally screen large libraries and identify functional variants.