Modified CRISPR/Cas9 mediated generation of two MKK7 knockout human embryonic stem cell lines
oleh: C.H. Cole Sims, Matias I. Autio, Adriana Buskin, Christine Cheung, Rakesh Heer, Roger S.Y. Foo, Xin Wang
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2021-04-01 |
Deskripsi
Two cell lines were generated by CRISPR/Cas9 mediated knockout of MKK7 (MAP2K7) by removal of exon 1 or exons 4 through 7. These knockouts were confirmed at the transcript and protein levels. These hESCs are pluripotent and maintain tri-lineage differentiation capacity. These cell lines are a useful resource for studying MKK7 function in humans.