Using a different growth medium greatly improves distinction of Butyrivibrio fibrisolvens and Pseudobutyrivibrio xylanivorans strains by the cellular fatty acids and aldehydes profiles
oleh: Maša ZOREC, Maša VODOVNIK, Romana MARINŠEK LOGAR
| Format: | Article |
|---|---|
| Diterbitkan: | University of Ljubljana Press (Založba Univerze v Ljubljani) 2011-07-01 |
Deskripsi
A total of 11 ruminal strains currently assigned to Butyrivibrio fibrisolvens and Pseudobutyrivibrio xylanivorans were cultivated in two different media, rumen fluid containing M2 and short-chain fatty acid (SCFA) containing M330, and their cellular fatty acid methyl esters (FAME) and dimethylacetals (DMA) were analyzed using gas chromatography. A comparison of the FAME/DMA compositions revealed that the difference in SCFA contents in the growth medium induced a pronounced quantitative effect on the cellular branched-chain fatty acid and aldehydes proportions only in the P. xylanivorans strains. This study shows that FAME/DMA analysis is a powerful chemotaxonomic tool in the group of phenotypically similar rumen butyrivibria especially when the influence of the growth medium is evaluated.