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The possibility of developing transgenic indica rices through Agrobacterium –mediated transformation in the absence ofAcetosyringone at bacterial preinduction or cocultivation or both stages was assessed. Six weeks old, scutellum derived calluses ofindica rice viz., ASD 16, White Ponni, Pusa Basmati, Pusa Sugandh 4, Pusa Sugandh 5 were cocultivated with A.tumifaciensstrain EHA 105, harbouring the binary vector pCAMBIA 1305.1 with the β-glucouronidase (GUS) and hygromycin phosphotransferase genes in the T-DNA region. Addition of Acetosyringone (AS) to the pre induction medium and cocultivation mediuminduced higher levels of transient GUS expression than that obtained with the addition of AS to either of the stages. Adddition ofsucrose to both the media revealed that the transient expression levels were similar to those obtained by the addition of AS. Theresultant fertile plants were stable ttransformants as revealed by GUS histochemical assay and PCR analysis for the GUS and HPTgenes. The results indicated that the addition of phenolics like AS may not be essential for the induction of vir genes anddevelopment of transgenic indica rices are also possible under AS free conditions.