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Organotin(IV) complexes recently have been receiving great attention due to their stability with a unique structure, physical and chemical properties. There are many applications, the organotin(IV) can be used as catalysts, antifouling agents, UV- and heat stabilizers, anticorrosion, anticancer and antimicrobial activities. This review summarizes the synthesis methods, characterization and biological activities of organotin(IV) thiosemicarbazones derivatives with their activities as anticancer and antimicrobial agents.