Preparation of long single-strand DNA concatemers for high-level fluorescence in situ hybridization
oleh: Dongjian Cao, Sa Wu, Caili Xi, Dong Li, Kaiheng Zhu, Zhihong Zhang, Hui Gong, Qingming Luo, Jie Yang
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2021-10-01 |
Deskripsi
Cao et al. present a method to prepare long single-strand DNA concatemers (lssDNAc) through a controllable rolling-circle amplification (CRCA), used to develop AmpFISH workflows. Their method is suitable for labelling to both individual mRNA molecules and chromosomes and can also be multiplexed with immunofluorescence, allowing for sensitive RNA/DNA detection.