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Background: The only source of cornea for corneal transplantation is from timely harvested safe eyes of deceased donors. Nurses attending on terminally ill patients can, if trained, motivate the relatives for eye donation. Aims: The aim of this study is to determine impact of an educational lecture on knowledge and attitude of nursing students toward eye donation. Settings and Design: This was an interventional study. Subjects and Methods: Nursing students in their 1st year and 4th year of training were administered a prestructured questionnaire. A 30-min interactive session was conducted for them regarding eye donation and transplantation. After 4 months, the questionnaire was readministered and their responses were analyzed with regard to number of correct responses for each question. Statistical Analysis Used: The statistical analysis used in this study was MedCalc for Windows, version 13.3.1 (MedCalc Software, Ostend, Belgium). Student's t-test was used to analyze change in knowledge and attitude. P <0.05 was considered significant. Results: The difference in the level of knowledge and attitude between first year and final year students before the exposure to educational awareness video was not statistically significant (P = 0.2 and P = 0.9, respectively). Four months following the interactive video, change in knowledge levels was significant for both groups (P = 0.01 - first year and P = 0.006 - final year), but there was no significant change in their attitude toward eye donation. Conclusion: Inclusion of eye donation in nursing curriculum is likely to be effective in imparting knowledge to nursing students who may be potential counselors for eye donation. “The proof of the pudding lies in the eating” and whether this translates to action by actual increase in registration as donors and corneal harvests remains to be seen.