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Objective: Complications during laparoscopic surgery involving the bladder, bowel, and major vessels have been reported extensively. However, uterine manipulator-associated injuries are seldom reported. Case Report: We describe herein the case of a 28-year-old female patient who underwent a laparoscopic cystectomy 5 days after cervical conization, during which the uterine manipulator perforated the anterior cul-de-sac through the cervix. Fortunately, the wound healed with conservative treatment and no adverse consequences were found. Conclusion: This case serves to highlight the potential for complications following seemingly benign maneuvers.