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A total of 2340 mammary glands of 585 primiparous and pluriparous lactating buffaloes in different stages of lactation were examined with California Mastitis Test ( CMT) and laboratory examination was carried out to identify the most prevalent micro-organisms in clinical and sub-clinical mastitis. The physical examination revealed 2.61 per cent blind teats and CMT revealed 6.71 per cent positive quarters for mastitis. Microbiological examination of 157 sub-clinical mastitis milk samples and 46 clinical mastitis milk samples was carried out. There was high occurrence of streptococci (35.46%) followed by staphylococci (33.99%), E. coli (27.09%), pseudomonas spp. ( 1.97%) and Corynaebacterium pyogenes (1.48%). The in vitro sensitivity revealed kanamyciin (82.6%), highly effective against various isolates followed by gentamycin (53.0%).