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Isolation and Molecular Identification of <i>Serratia</i> Strains Producing Chitinases, Glucanases, Cellulases, and Prodigiosin and Determination of Their Antifungal Effect against <i>Colletotrichum siamense</i> and <i>Alternaria alternata</i> In Vitro and on Mango Fruit
oleh: J. Alexander Trejo-López, Esmeralda Rangel-Vargas, Carlos A. Gómez-Aldapa, José R. Villagómez-Ibarra, Reyna N. Falfán-Cortes, Otilio A. Acevedo-Sandoval, Javier Castro-Rosas
Format: | Article |
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Diterbitkan: | MDPI AG 2022-08-01 |
Deskripsi
Microorganisms represent a viable option for the control of phytopathogens. From the surface of healthy mangoes, different bacteria were isolated. For all isolated bacterial strains, we determined their antimicrobial activity against a fungal strain that caused anthracnose in mangoes and against <i>Alternaria alternata</i>, both in the culture medium and directly on mangoes. The bacterial strains with the highest antifungal activity were identified by sequencing the 16s rRNA gene. Two species of <i>Serratia</i> were identified: <i>marcescens</i> and <i>nematodiphila</i>. Finally, the chitinolytic, glucanolytic, and cellulolytic activity and prodigiosin production of bacteria with antifungal activity was determined. Five fungal strains were isolated from mangoes with anthracnose. Only one strain was responsible for anthracnose in mangoes. This fungal strain was identified as <i>Colletotrichum siamense</i>. Against <i>C. siamense</i> and <i>A. alternata</i> in vitro and in mango selected strains of <i>Serratia</i> showed antifungal activity. Finally, the <i>Serratia</i> strains produced chitinases, glucanases, cellulases and prodigiosin, and the two <i>S. marcescens</i> strains did not produce hemolysins. The three <i>Serratia</i> strains isolated in this study can potentially be used in the biological control of anthracnose caused by <i>C. siamense</i> and <i>A. alternata</i> on mango.