Determinação rápida de fármacos básicos em plasma por cromatografia a gás com detector de nitrogênio e fósforo
oleh: Lilian Feltraco Lizot, Luana Letícia da Silva, Bárbara Spaniol, Marina Venzon Antunes, Rafael Linden, Rafael Lanaro
| Format: | Article |
|---|---|
| Diterbitkan: | Sociedade Brasileira de Química 2012-01-01 |
Deskripsi
A simple and fast method for determination of 40 basic drugs in human plasma employing gas-chromatography with nitrogen-phosphorus detection was developed and validated. Drugs were extracted from 800 µL of plasma with 250 µL of butyl acetate at basic pH. Aliquots of the organic extract were directly injected on a column with methylsilicone stationary phase. Total chromatographic run time was 25 min. All compounds were detected in concentrations ranging from therapeutic to toxic levels, with intermediate precision CV% below 11.2 and accuracy in the range of 92-114%.