Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination
oleh: Behnam Enghiad, Chunshuai Huang, Fang Guo, Guangde Jiang, Bin Wang, S. Kasra Tabatabaei, Teresa A. Martin, Huimin Zhao
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2021-02-01 |
Deskripsi
Direct cloning of biosynthetic gene clusters is difficult due to their size and repetitive nature. Here, the authors present CAPTURE, which uses Cas12a and in vivo Cre-lox recombination for efficient cloning of gene clusters up to 113 kb, and demonstrate how this method can be used for large-scale discovery of novel natural products.