Orthogonal glycolytic pathway enables directed evolution of noncanonical cofactor oxidase
oleh: Edward King, Sarah Maxel, Yulai Zhang, Karissa C. Kenney, Youtian Cui, Emma Luu, Justin B. Siegel, Gregory A. Weiss, Ray Luo, Han Li
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2022-11-01 |
Deskripsi
Engineering enzymes to accept noncanonical cofactor biomimetics is difficult. Here, the authors establish a self-sufficient growth selection method and demonstrate its application in engineering the Lactobacillus pentosus NADH oxidase to efficiently recycle reduced nicotinamide mononucleotide (NMNH).