Prolonged DNA hydrolysis in water: A study on DNA stability
oleh: Paolo Fattorini, Giorgio Marrubini, Serena Bonin, Barbara Bertoglio, Pierangela Grignani, Elisa Recchia, Paola Pitacco, Francesca Procopio, Carolina Cantoni, Irena Zupanič Pajnič, Solange Sorçaburu-Cigliero, Carlo Previdere`
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2018-10-01 |
Deskripsi
This work provides a protocol for the in vitro production of damaged DNA samples. In particular, heat-mediated hydrolysis of the samples at 70 °C in ultrapure water was performed in 1.7 mL Eppendorf tubes sealed by Parafilm for 0–36 h. The chemical/physical features of the resulting samples are described. After normalization of the qPCR data, these were compared with those obtained from samples treated for 0–10 h in a previous study. Keywords: qPCR, Degraded samples, DNA quantification, DNA hydrolysis