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The purpose of this study was to describe morphological traits and estimate genetic divergence and parameters between accessions of the genus Heliconia sp. from different municipalities in the state of Mato Grosso, Brazil. A set of 25 traits, 15 quantitative and 10 qualitative, were evaluated. The genetic divergence was estimated based on Mahalanobis’ distance, with the clustering methods Unweighted Pair Group Method using Arithmetic Averages (UPGMA) and canonical variables. Genetic variability was observed for all assessed quantitative traits and the accessions were grouped in different classes. The traits with highest relative contribution to variability were longevity of flower stems and inflorescence length. The results indicated the existence of genetic variability among accessions of the Heliconia sp. germplasm bank, which can be exploited in breeding programs of the species anobis’ distance, with the clustering methods Unweighted Pair Group Method using Arithmetic Averages (UPGMA) and canonical variables. Genetic variability was observed for all assessed quantitative traits and the accessions were grouped in different classes. The traits with highest relative contribution to variability were longevity of flower stems and inflorescence length. The results indicated the existence of genetic variability among accessions of the Heliconia sp. germplasm bank, which can be exploited in breeding programs of the species.