Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture
oleh: Sara Pijuan-Galitó, Christoffer Tamm, Jens Schuster, Maria Sobol, Lars Forsberg, Catherine L. R. Merry, Cecilia Annerén
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2016-07-01 |
Deskripsi
Improved culture methods are needed to reliably grow human pluripotent stem cells (hPSCs) on a large scale. Here, the authors identify a xeno-free medium with a supplement of Inter-α-inhibitor that supports long-term propagation and improved single-cell passaging of hPSCs on uncoated plastic.