A HaloTag-TEV genetic cassette for mechanical phenotyping of proteins from tissues
oleh: Jaime Andrés Rivas-Pardo, Yong Li, Zsolt Mártonfalvi, Rafael Tapia-Rojo, Andreas Unger, Ángel Fernández-Trasancos, Elías Herrero-Galán, Diana Velázquez-Carreras, Julio M. Fernández, Wolfgang A. Linke, Jorge Alegre-Cebollada
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2020-04-01 |
Deskripsi
Testing mechanical forces on native molecules in natural environments remains a challenge. Here the authors engineer titin to carry a HaloTag-TEV insertion to allow analysis of dynamics under force in muscle fibers.