Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations

oleh: Nils Gustafsson, Siân Culley, George Ashdown, Dylan M. Owen, Pedro Matos Pereira, Ricardo Henriques

Format: Article
Diterbitkan: Nature Portfolio 2016-08-01

Deskripsi

Super-resolution fluorescent imaging typically makes use of intense phototoxic illumination. Here the authors achieve live-cell super-resolution imaging using low-illumination standard microscopes with the aid of a new analytical approach called Super-Resolution Radial Fluctuations (SRRF), provided as an ImageJ plugin.