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The extracellular matrix (ECM) surrounding cancer cells becomes stiffer during tumor progression, which influences cancer cell behaviors such as invasion and proliferation through modulation of gene expression as well as remodeling of the actin cytoskeleton. In this study, we show that <i>MMP24</i> encoding matrix metalloproteinase (MMP)-24 is a novel target gene of Yes-associated protein (YAP), a transcription coactivator known as a mechanotransducer. We first examined the effect of substrate stiffness on <i>MMP24</i> expression in MCF-7 human breast cancer cells and showed that the expression of <i>MMP24</i> was significantly higher in cells grown on stiff substrates than that on soft substrates. The <i>MMP24</i> expression was significantly reduced by knockdown of YAP. In contrast, the expression of constitutively active YAP increased <i>MMP24</i> promoter activity. In addition, binding of YAP to the <i>MMP24</i> promoter was confirmed by the chromatin immunoprecipitation (ChIP) assay. These results show that ECM stiffening promotes YAP activation, thereby inducing <i>MMP24</i> expression. Based on the Human Protein Atlas database, breast cancer patients with lower <i>MMP24</i> expression exhibit the worse survival rates overall. Thus, <i>MMP24</i> may negatively regulate the aggressiveness of cancer cells under the stiff ECM environment during tumor progression.