Engineered dual selection for directed evolution of SpCas9 PAM specificity
oleh: Gregory W. Goldberg, Jeffrey M. Spencer, David O. Giganti, Brendan R. Camellato, Neta Agmon, David M. Ichikawa, Jef D. Boeke, Marcus B. Noyes
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2021-01-01 |
Deskripsi
The PAM specificity of SpCas9 can be altered with positive selection during directed evolution. Here the authors use simultaneous positive and negative selection to improve activity on NAG PAMs while reducing activity on NGG PAMs.