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Imaging neuropeptide release at synapses with a genetically engineered reporter
oleh: Keke Ding, Yifu Han, Taylor W Seid, Christopher Buser, Tomomi Karigo, Shishuo Zhang, Dion K Dickman, David J Anderson
Format: | Article |
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Diterbitkan: | eLife Sciences Publications Ltd 2019-06-01 |
Deskripsi
Research on neuropeptide function has advanced rapidly, yet there is still no spatio-temporally resolved method to measure the release of neuropeptides in vivo. Here we introduce Neuropeptide Release Reporters (NPRRs): novel genetically-encoded sensors with high temporal resolution and genetic specificity. Using the Drosophila larval neuromuscular junction (NMJ) as a model, we provide evidence that NPRRs recapitulate the trafficking and packaging of native neuropeptides, and report stimulation-evoked neuropeptide release events as real-time changes in fluorescence intensity, with sub-second temporal resolution.