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An experiment was undertaken in the open field at thenursery of Hort. Res. Inst., ARC, Giza, Egypt during 2013 and 2014seasons to explore the effect of different water qualities (fresh, mixed (50%fresh + 50% sanitary water) and primary treated sanitary water), variousconcentrations of jasmine oil (0.0, 0.03 and 0.05%) applied as foliar spray,3 times with 15 days interval and their interactions on growth and chemicalcomposition of one-year-old seedlings of Australian pine (Casuarinaequisetifolia L.) and River Red Gum (Eucalyptus rostrata Schlecht.)cultivated in 16-cm-diameter black polyethylene bags filled with about 3.5kg of clayey soil.The obtained results have shown that all vegetative and root growthparameters of the used two trees were significantly increased in response tothe different sole treatments and interactions used in this study. A similartrend was also obtained concerning the leaf content of chlorophyll a, b andcarotenoids, as well as total soluble sugars, N, P and K in the stem, leavesand roots, as they were markedly increased by the various employedtreatments. On the other hand, content of Pb and Cd in the stem, leaves androots was increased as the level of sanitary water increased in irrigationwater, but they were gradually decreased with increasing jasmine oilconcentration in spray solution. In general, content of these two metals invarious plant organs was greatly less than their critical levels that inducetoxicity. So, no morpho-or physio-logical disorders appear on the plants ofthe two studied tree species. However, the prevalence in all previousmeasurements was for the combining between irrigation with mixed waterand spraying with 0.05% jasmine oil solution, as this combination gave thebest rate of vegetative and root growth and the highest content of pigments,total soluble sugars, N, P and K in plant tissues accompanied with the leastcontent of Pb and Cd elements.Hence, it could be said that primary treated sanitary water can be usedafter mixing with fresh water at equal parts in irrigation Casuarinaequisetifolia L. and Eucalyptus rostrata Schlecht. plants proved sprayingthem with 0.05% jasmine oil solution, 3 times with 15 days interval toachieve the best growth and highest quality.