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<i>Micrococcus porci</i> sp. nov., Isolated from Feces of Black Pig (<i>Sus scrofa</i>)
oleh: Ai-Yun Lee, Chia-Hsuan Chen, Jong-Shian Liou, Yu-Chun Lin, Moriyuki Hamada, Yu-Ting Wang, Lin-Liang Peng, Shen-Chang Chang, Chih-Chieh Chen, Chuen-Fu Lin, Lina Huang, Chien-Hsun Huang
Format: | Article |
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Diterbitkan: | MDPI AG 2022-10-01 |
Deskripsi
An aerobic bacterium, designated as strain KD337-16<sup>T</sup>, was isolated from the fecal samples of a black pig. It exhibited spherical, non-motile and non–spore-forming, Gram-positive cells. KD337-16<sup>T</sup> was identified as a member of the genus <i>Micrococcus</i> through 16S rRNA gene sequencing, and its closest relatives were found to be <i>Micrococcus endophyticus</i> YIM 56238<sup>T</sup> (99.5% similarity), <i>Micrococcus luteus</i> NCTC 2665<sup>T</sup> (99.1%), <i>Micrococcus yunnanensis</i> YIM 65004<sup>T</sup> (99.1%), <i>Micrococcus aloeverae</i> AE-6<sup>T</sup> (99.1%), <i>Micrococcus antarcticus</i> T2<sup>T</sup> (98.9%), and <i>Micrococcus flavus</i> LW4<sup>T</sup> (98.7%). Phylogenomic trees were constructed, and strain KD337-16<sup>T</sup> was found to form its own cluster as an independent lineage of <i>M. flavus</i> LW4<sup>T</sup>. Between KD337-16<sup>T</sup> and its close relatives, the average nucleotide identity, average amino acid identity, and digital DNA–DNA hybridization were below the respective species delineation thresholds at 82.1–86.6%, 78.1–86.1%, and 24.4–34.9%. The major cellular fatty acids and polar lipids were anteiso-C<sub>15:0</sub> and iso-C<sub>15:0</sub>, and DPG and PG, respectively. The predominant menaquinone was MK-8(H<sub>2</sub>). Taken together, the results indicate that strain KD337-16<sup>T</sup> is a novel species of the genus <i>Mic</i><i>rococcus,</i> for which the name <i>Micrococcus</i> <i>porci</i> sp. nov. is proposed. The type strain is KD337-16<sup>T</sup> (=BCRC 81318<sup>T</sup> = NBRC 115578<sup>T</sup>).