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An experiment was carried out to investigate the standardization of harvest maturity indicators in Jackfruit (Artocarpus heterophyllus Lam.)The mean number of spines/cm2 was lowest in tree 1 (8.0), the mean metallic sound (hedonic scale) was highest in tree 1 (2.8), the mean fruit length was significantly increasing and reaching its maximum in tree 1 (39.00 cm), and the mean fruit circumference was significantly increasing and reaching its maximum in tree 1 (39.00 cm) (41.48 cm).The experiment's data were considered non-replicated, and the recorded data were statistically analyzed using a one-way ANOVA design in the computer software MS Excel. Considering morphological analysis the characters viz.,  fruit circumference(39.00 cm), low spine density(8.0), moderate to high spreading of spines, presence of sensible hollow metallic sound could be used as the maturity indices of jackfruit. It is also noted that jackfruit could be harvested after 100 days of fruit set.