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In this paper, we show that biharmonic hypersurfaces with at most two distinct principal curvatures in pseudo-Riemannian space form Nsn+1c with constant sectional curvature c and index s have constant mean curvature. Furthermore, we find that such biharmonic hypersurfaces Mr2k−1 in even-dimensional pseudo-Euclidean space Es2k, Ms−12k−1 in even-dimensional de Sitter space Ss2kcc>0, and Ms2k−1 in even-dimensional anti-de Sitter space ℍs2kcc<0 are minimal.