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The experiment developed the ultra-high-performance liquid chromatography−tandem mass spectrometry (UPLC/MS/MS) method for testing emamectin benzoate, and studied the metabolism of emamectin benzoate in rice plants and rice-growing environments via application of this testing method. The dissipation curve of emamectin benzoate standard substance was good at 0.5−200 μg L^−1, and its correlation coefficient was greater than 0.99. In the concentration range of 0.1−50 μg kg^−1, the average recovery rate of plants, soil, and field water was 82 %−102 %, and relative standard deviation (RSD) was between 0.3 % and 15.9 %. Half-lives in rice plants and soil were 0.8−2.8 days and 1.9−3.8 days, respectively, and emamectin benzoate was not detected in rice or rice hull. The experiment showed that emamectin benzoate is harmless to human health at the concentration recommended by the manufacturer.