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Glyphosate is the most widely used herbicide for weed control in citrus orchards in Brazil; therefore, it is likely that several species have gained resistance to this herbicide and that more than one resistant species can be found in the same orchard. The objective was to identify weeds resistant to glyphosate in citrus orchards from different regions of the São Paulo State (SP) and determine how many resistant species are present within the same orchard. Seeds of <i>Amaranthus deflexus</i>, <i>A. hybridus</i>, <i>Bidens pilosa</i>, <i>Chloris elata</i>, <i>Conyza bonariensis</i>, <i>Digitaria insularis</i>, <i>Solanum Americanum</i>, and <i>Tridax procumbens</i>, which, as reported by growers, are suspected to be resistant to glyphosate, were collected from plants that survived the last application of this herbicide (>720 g of acid equivalent [ae] ha^–1) in sweet orange and Tahiti acid lime orchards. Based on dose–response and shikimic acid accumulation assays, all populations of <i>A. deflexus</i>, <i>A. hybridus</i>, <i>B. pilosa</i>, and <i>T. procumbens</i> were sensitive to glyphosate. However, populations of <i>B. pilosa</i> from the Olimpia region (R-NS, R-PT and R-OdA) showed signs of resistance based on plant mortality rates by 50% within a population (LD₅₀ = 355–460 g ae ha⁻¹). All populations of <i>C. bonariensis</i>, <i>C. elata</i>, and <i>D. insularis</i> were resistant to glyphosate, presenting resistance ratios from 1.9 to 27.6 and low shikimate accumulation rates. <i>Solanum americanum</i> also showed resistance, with resistance ratios ranging from 4.3 to 25.4. Most of the citrus orchards sampled presented the occurrence of more than one species resistant to glyphosate: Nossa Senhora—one species; Olhos D’agua and Passatempo—two species; Araras—four species; and Cordeiropolis and Mogi-Mirim—up to five species. The results reported in this paper provide evidence of multiple species in citrus orchards from São Paulo that have exhibited resistance to glyphosate. This underscores the difficulties in managing glyphosate-resistant weeds which are prevalent throughout the country, such as <i>C. bonariensis</i> and <i>D. insularis</i>. The presence of these resistant species further complicates the control of susceptible species that may also develop resistance. In addition, the glyphosate resistance of <i>S. americanum</i> was identified for the first time.