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This study aims to discuss the synthesis and fabrication of SnO₂-In₂O₃-based thick-films and their biosensing applications. The structural characterization of SnO₂-In₂O₃ nanocomposites was performed using X-ray diffraction, Raman spectroscopy and transmission electron microscopy. Furthermore, the screen-printing technology was used in the fabrication of conductive electrodes to form an interdigitated capacitive structure, and the sensor layer based on the mixture of SnO₂ and In₂O₃. Moreover, the sensing performance of the developed structure was tested using <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) and <i>Staphylococcus aureus</i> (<i>S. aureus</i>) bacteria. In addition, the validation of sensing characteristics was performed by electrochemical impedance spectroscopic and self-resonant frequency analysis. Finally, the sensing properties were analyzed for two consecutive days, and changes in both <i>P. aeruginosa</i> and <i>S. aureus</i> pathogens growing media were also studied.