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<i>Phytophthora capsici</i> causes destructive disease in several crop species, including pepper (<i>Capsicum annuum</i> L.). Resistance in this species is physiologically and genetically complex due to many <i>P. capsici</i> virulence phenotypes and different QTLs and R genes among the identified resistance sources. Several primer pairs were designed to follow an SNP (G/A) within the <i>CA_011264</i> locus linked to the <i>Pc5.1</i> locus. All primer pairs were designed on DNA sequences derived from <i>CaDMR1</i>, a homoserine kinase (HSK), which is a gene candidate responsible for the major QTL on chromosome <i>P5</i> for resistance to <i>P. capsici</i>. A panel of 69 pepper genotypes from the Southern Seed germplasm collection was used to screen the primer pairs designed. Of these, two primers (<i>Phyto</i>_for_2 and <i>Phyto</i>_rev_2) surrounding the SNP proved successful in discriminating susceptible and resistant genotypes when combined with a restriction enzyme (<i>BtgI</i>). This new marker (called <i>Phyto</i>) worked as expected in all genotypes tested, proving to be an excellent candidate for marker-assisted selection in breeding programs aimed at introgressing the resistant locus into pure lines.