Find in Library

Modern agricultural cultivation relies heavily on genetically modified plants that survive after exposure to herbicides that kill weeds. Despite this biotechnology, there is a growing need for new sustainable, environmentally friendly, and biodegradable herbicides. We developed a novel [CuL₂]Br₂ complex (L = bis{4H-1,3,5-triazino[2,1-b]benzothiazole-2-amine,4-(2-imidazole) that is active on PSII by inhibiting photosynthetic oxygen evolution on the micromolar level. [CuL₂]Br₂ reduces the F_V of PSII fluorescence. Artificial electron donors do not rescind the effect of [CuL₂]Br₂. The inhibitory mechanism of [CuL₂]Br₂ remains unclear. To explore this mechanism, we investigated the effect of [CuL₂]Br₂ in the presence/absence of the well-studied inhibitor DCMU on PSII-containing membranes by OJIP Chl fluorescence transient measurements. [CuL₂]Br₂ has two effects on Chl fluorescence transients: (1) a substantial decrease of the Chl fluorescence intensity throughout the entire kinetics, and (2) an auxiliary “diuron-like” effect. The initial decrease dominates and is observed both with and without DCMU. In contrast, the “diuron-like” effect is small and is observed only without DCMU. We propose that [CuL₂]Br₂ has two binding sites for PSII with different affinities. At the high-affinity site, [CuL₂]Br₂ produces effects similar to PSII reaction center inhibition, while at the low-affinity site, [CuL₂]Br₂ produces effects identical to those of DCMU. These results are compared with other PSII-specific classes of herbicides.