Enrichment and identification of Δ9-Tetrahydrocannabinolic acid synthase from Pichia pastoris culture supernatants
oleh: Kerstin Lange, Ansgar Poetsch, Andreas Schmid, Mattijs K. Julsing
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2015-09-01 |
Deskripsi
This data article refers to the report Δ9-Tetrahydrocannabinolic acid synthase (THCAS) production in Pichia pastoris enables chemical synthesis of cannabinoids (Lange et. al. 2015) [2]. THCAS was produced on a 2 L lab scale using recombinant P. pastoris KM71 KE1. Enrichment of THCAS as a technically pure enzyme was realized using dialysis and cationic exchange chromatography. nLC-ESI-MS/MS analysis identified THCAS in different fractions obtained by cationic exchange chromatography.