Simultaneous Intranasal/Intravascular Antibody Labeling of CD4+ T Cells in Mouse Lungs
oleh: Yanqun Wang, Jing Sun, Rudragouda Channappanavar, Jingxian Zhao, Stanley Perlman, Jincun Zhao
| Format: | Article |
|---|---|
| Diterbitkan: | Bio-protocol LLC 2017-01-01 |
Deskripsi
CD4+ T cell responses have been shown to be protective in many respiratory virus infections. In the respiratory tract, CD4+ T cells include cells in the airway and parenchyma and cells adhering to the pulmonary vasculature. Here we discuss in detail the methods that are useful for characterizing CD4+ T cells in different anatomic locations in mouse lungs.