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<i>Colchicum pusillum</i> Sieber is a plant from Colchicaceae family that particularly rich in tropolonic alkaloids, colchicine. The aim of the study is to determine the apoptosis induction effect of <i>Colchicum pusillum</i> extract in Colo-741, metastatic colon cancer cells and if so, to determine by which apoptotic pathway. <i>Colchicum pusillum</i> was collected and extracted with ethanol. Apoptotic activities of <i>Colchicum pusillum</i> were investigated by immunocytochemistry using antibodies directed against to caspase-3, cytochrome-c and FasLigand (FasL) in Colo-741 cells. Immunocytochemical staining results stated that <i>Colchicum pusillum</i> extract at 20 µg/mL dose, increased caspase-3 and FasL activity significantly but was not able to activate cytochrome-c activity. The results suggested that <i>Colchicum pusillum</i> extract induced apoptosis in metastatic colon cancer cells via extrinsic apoptotic pathway.