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Members of the <i>Mi</i><i>14-3-3</i> gene family interact with target proteins that are widely involved in plant hormone signal transduction and physiology-related metabolism and play important roles in plant growth, development and stress responses. In this study, <i>14-3-3</i><i>s</i> family members are identified by the bioinformatic analysis of the mango (<i>Mangifera indica</i> L.) genome. The gene structures, chromosomal distributions, genetic evolution, and expression patterns of these genes and the physical and chemical properties and conserved motifs of their proteins are analysed systematically. The results identified 16 members of the <i>14-3-3</i> genes family in the mango genome. The members were not evenly distributed across the chromosomes, and the gene structure analysis showed that the gene sequence length and intron number varied greatly among the different members. Protein sequence analysis showed that the Mi14-3-3 proteins had similar physical and chemical properties and secondary and tertiary structures, and protein subcellular localization showed that the Mi14-3-3 family proteins were localized to the nucleus. The sequence analysis of the <i>Mi14-3-3s</i> showed that all Mi14-3-3 proteins contain a typical conserved PFAM00244 domain, and promoter sequence analysis showed that the <i>Mi14-3-3</i> promoters contain multiple hormone-, stress-, and light-responsive cis-regulatory elements. Expression analysis showed that the <i>14-3-3</i> genes were expressed in all tissues of mango, but that their expression patterns were different. Drought, salt and low temperature stresses affected the expression levels of <i>14-3-3</i> genes, and different <i>14-3-3</i> genes had different responses to these stresses. This study provides a reference for further studies on the function and regulation of <i>Mi</i><i>14-3-3</i> family members.