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Tuberculosis is the most important zoonotic bacterial disease in nonhuman primates. The present study investigated 27 carcasses of non human primates of Assam State Zoo and Department of Forest and Environment, Govt. of Assam during the period from August 2007 to November 2009. Six positive tuberculosis cases were recorded. For detection of antibodies to Mycobacterium tuberculosis and Mycobacterium bovis, three serum samples of free ranging non human primates of Kaziranga National Park were tested by using PrimaTB STAT-PAK Assay kit and all three samples showed negative result. The study suggests that captive non human primates should be screened regularly to control the spread of the diseas