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The determination of urease activity is one of the important projects in 《Enzyme engineering experiment》 and production. In the paper, the urease activity of soybean was determined by pH increment method, titration method and Nash reagent method, and the three methods were compared. The results showed that the urease activity of the same sample measured by pH increment method, titration method and Nash reagent method was not equal in value and could not be used mutually, and the urease activity of the same sample measured by Nash reagent method was the highest. The same experimental project adopts a variety of methods to improve students ’ interest in learning, improve students’ data analysis ability, and provide a better way to cultivate applied and innovative talents.