CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation
oleh: Rubina Tuladhar, Yunku Yeu, John Tyler Piazza, Zhen Tan, Jean Rene Clemenceau, Xiaofeng Wu, Quinn Barrett, Jeremiah Herbert, David H. Mathews, James Kim, Tae Hyun Hwang, Lawrence Lum
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2019-09-01 |
Deskripsi
CRISPR-Cas9 genome editing is presumed to knock out gene function by generating a frameshift during NHEJ repair. Here, the authors investigate mRNA and protein expression in edited lines and find genome editing can generate internal ribosome entry sites or alternatively spliced variants.