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Anammox activity of water and sediment taken from marine ecosistem around Semarang were investigated in anammox media agar and batch reactor. Effect of increasing salinities (3% to 5%, 7% and 9%) and decreasing salinities (3% to 2%, 1% and 0%) were  investigated. Water and sediment taken from marine ecosistem around Semarang city showed a positive result. Increasing salinity up to 9% will have a positive influence on the activity of anammox bacteria. Whereas, the decrease in salinity will negatively affect anammox bacteria. The ratio of ammonium:nitrite for anammox process ranges from 0.89 to 1.22 and ammonium removal rate varies from 0.08 to 0.59 mg-N/liter hour.