Proteomics dataset from 26th Dynasty Egyptian mummified remains sampled using minimally invasive skin sampling tape strips

oleh: Dylan H. Multari, Prathiba Ravishankar, Geraldine J. Sullivan, Ronika K. Power, Constance Lord, James A. Fraser, Paul A. Haynes

Format: Article
Diterbitkan: Elsevier 2022-12-01

Deskripsi

Paleoproteomics typically involves the destructive sampling of precious bioarchaeological materials. This analysis aims to investigate the proteins identifiable via nanoLC-MS/MS from highly degraded 26th Dynasty Egyptian mummified human remains (NMR.29.1-8) after non-destructive sampling with commercially available dermatology-grade skin sampling tape strips. A collection of cranial and other bone fragments were sampled with the tape strips then subsequently analysed using a shotgun proteomics approach. The number of proteins identified using this method ranged from 18 to 437 at a peptide FDR of <1%. Deamidation ratios were assessed using an in-house R script, with asparagine deamidation averaging ∼20–30% and glutamine deamidation averaging ∼15–25%.