Find in Library

A fully identified marine-derived Penicillum spinulosum MH2 was isolated from the Eastern Harbor, Egypt. Data confirmed that PDB exhibited the highest fungal biomass 8.31 g/L after 15 days of incubation. The SEM photographs of prepared chitosan showed thick and rough surface morphology. The absorption bands of the extracted chitosan detected by FTIR were identical to those of the standard one. The 1H NMR spectrum of extracted chitosan showed two sets of singlet peaks at 2.46–2.99 and 2.64–2.67 ppm, which were assigned to the (H-2) protons of different chemical environments as in the structure of chitosan; one adjacent to the primary amine (2.46–2.99 ppm) and the other adjacent to acetyl group (2.64–2.67 ppm). The spectrum also showed broad signal of overlapped multiplets peaks (3.05–3.83) ppm, which correspond to the protons (H-3, H-4, H-5, and H-6). In addition, the results supported the extracted chitosan efficacy as antimicrobial agent against several microbes (S. aureus, B. subtilis, F. solani, R. solani, P. nutatum, and C. albicans). Moreover, it exhibited considerable antifouling activity against fouling bacteria. Keywords: Penicillum spinulosum, Chitosan, Antimicrobial, Antifouling activity